Application of BIOMED-2 primers in analysis of T-cell receptor gamma gene rearrangements in paraffin-embedded tissue specimens of T-cell lymphoma / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 253-257, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-319715
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the practical values of PCR detectable T-cell receptor (TCR) gene rearrangement in paraffin embedded tissue samples in the diagnosis of T-cell malignancies using BIOMED-2 PCR multiplex tubes TCRgamma(A+B).</p><p><b>METHODS</b>Traditional phenol-chloroform method was used to extract DNA from 55 cases of archival paraffin embedded tissues samples of T-cell malignancies and the DNA quality was evaluated by PCR-based amplification of housekeeping gene beta-globin. The selected BIOMED-2 PCR multiplex tubes TCRgamma(A+B) were used to detect TCR gene rearrangement and comparison with the results of universal TCR primers (T(VG)/T(JX)) was performed.</p><p><b>RESULTS</b>Positive detection rates by the BIOMED-2 multiplex tubes TCRgamma(A+B) and the universal primers (T(VG)/T(JX)) were 76.4% and 60.0%, respectively. There were not statistical difference between the methods (P > 0.05).</p><p><b>CONCLUSION</b>BIOMED-2 multiplex tubes TCRgamma(A+B) is suitable for detection of clonal rearrangements of TCR genes in current archival paraffin embedded tissue samples of T-cell malignancies.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
DNA, Neoplasm
/
Polymerase Chain Reaction
/
Lymphoma, T-Cell
/
Paraffin Embedding
/
Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
/
DNA Primers
/
Beta-Globins
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2009
Type:
Article
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