Effect of benzo(a)pyrene on platelet aggregation and expression of P-selectin / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 51-56, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-319833
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of benzo(a)pyrene (BaP) on platelet aggregation and expression of P-selectin.</p><p><b>METHODS</b>Blood samples were collected from healthy volunteers and the platelets was washed. Platelet aggregation was monitored by aggregometer and the expression of P-seletin was detected by whole blood flow cytometry.</p><p><b>RESULT</b>BaP (10 μmol/L, 1 μmol/L and 0.1 μmol/L) did not induce platelet aggregation; however, preincubation with BaP (10 μmol/L) significantly enhanced ADP-induced platelet aggregation (P < 0.01) and platelet aggregation was (80 ± 10)%, while BaP-preincubation failed to enhance platelet aggregation under collagen and thrombin stimulation. Flow cytometry showed that preincubation with BaP increased ADP-induced, but not thrombin-induced P-selectin expression (P < 0.01).</p><p><b>CONCLUSION</b>BaP can stimulate ADP-induced platelet aggregation and P-selectin expression, probably through the interaction with ADP-mediated signal pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Benzo(a)pyrene
/
Blood
/
Blood Platelets
/
Thrombin
/
Adenosine Diphosphate
/
Platelet Aggregation
/
Collagen
/
P-Selectin
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2011
Type:
Article
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