Detection of RRM1, ERCC1 and BRCA1 gene expression in non-small cell lung cancer tissues and peripheral blood by SYBR real-time fluorescent quantitative PCR / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 628-633, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-319847
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for the detection of RRM1, ERCC1 and BRCA1 gene expression by SYBR real-time fluorescent quantitative PCR in non-small cell lung cancer tissues and peripheral blood.</p><p><b>METHODS</b>The plasmid standard of RRM1, ERCC1, BRCA1 and β-actin genes was constructed. SYBR real-time PCR was performed, and the standard curve was established. The expressions of RRM1, ERCC1 and BRCA1 mRNA in non-small cell lung cancer tissues and peripheral blood were detected.</p><p><b>RESULT</b>The standard curve presented linearity. The liquate curves of standard gene were all single apex, indicating that a good specificity was obtained.</p><p><b>CONCLUSION</b>The developed SYBR real-time fluorescent quantitative PCR has advantage of convenient operation, low cost, good specificity and high veracity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
RNA, Messenger
/
Polymerase Chain Reaction
/
Actins
/
Carcinoma, Non-Small-Cell Lung
/
BRCA1 Protein
/
Tumor Suppressor Proteins
/
DNA-Binding Proteins
/
Endonucleases
/
Genetics
Type of study:
Diagnostic study
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2010
Type:
Article
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