A proteomic study of peripheral blood mononuclear cells in patients undergoing cardiopulmonary bypass / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 470-476, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-319874
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of cardiopulmonary bypass (CPB) on the profile of protein expression in peripheral blood mononuclear cells (PBMCs).</p><p><b>METHODS</b>Eleven patients undergoing cardiac surgery under cardiopulmonary bypass were enrolled in the study. Peripheral blood samples were collected before CPB (T0), 1 h after CPB (T1) and at the end of operation (T2), and PBMCs were obtained by gradient centrifugation. The profile of protein expression was analyzed using 2-D gel electrophoresis (2-DE) and mass spectrometry. The candidate proteins were further identified by Western blotting.</p><p><b>RESULT</b>Compared to protein profile at T0, 12 protein spots were identified to be up-regulated in PBMCs at T1 (P <0.05), among which S100A9 reached the peak level at T1 and decreased after operation,but not returned to its initial level.</p><p><b>CONCLUSION</b>Results indicate that 12 proteins are likely to be involved in CPB, however, their roles need to be elucidated.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Mass Spectrometry
/
Leukocytes, Mononuclear
/
Cardiopulmonary Bypass
/
Electrophoresis, Gel, Two-Dimensional
/
Proteome
/
Proteomics
/
Metabolism
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2010
Type:
Article
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