Measurement of differential values of inhaled volumes and exhaled volumes in mice / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the differential value of inhaled volume and exhaled volume in anesthesia mice, and its effect on the pressure change inside of plethysmograph.</p><p><b>METHODS</b>Pressure and temperature in head chamber of double-chamber plethysmograph were tested when mice were placed in the body chamber. The differential values of inhaled volume and exhaled volume of mice were calculated.</p><p><b>RESULT</b>The baseline pressure in head chamber increased with temperature rising within 12 minutes, then went down though temperature remained high. The inhaled volumes of 8 mice were (0.2842 + or - 0.0173)ml,and the differential value of inhaled volume and exhaled volume was (0.0012 + or - 0.0002)ml.</p><p><b>CONCLUSION</b>The inhaled volume is larger than exhaled volume, which makes pressure baseline of plethysmograph decreased. The differential value may be generated from respiratory quotient.</p>