Effects of paclitaxel loaded-drug micelles on cell proliferation and apoptosis of human lung cancer A549 cells / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1240-1245, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-320095
ABSTRACT
This study was conducted to investigate the paclitaxel loaded by hydrazone bonds in poly(ethylene glycol)-poly(caprolactone) micelles (mPEG-PCL-PTX) on proliferation and apoptosis of human lung cancer A549 cells and its possible mechanisms of anti-tumor activity. The cell proliferation was measured with MTT assay. Flow cytometry were used to analyze the cell cycle. The cell apoptosis was analyzed using Hoechst/P staining. The expression levels of apoptotic genes expression in the mitochondrial apoptosis pathway were detected by RT-PCR and Western blotting, respectively. The mPEG-PCL-PTX could inhibit the proliferation of A549 cells and promote the apoptosis. The Bax, caspase-3 protein expression were increased while Bcl-2 protein expression was decreased in A549 cells. Results showed that the polymer containing hydrazone bond is non-toxic in vitro, the mPEG-PCL-PTX micelles can inhibit the proliferation and induce the apoptosis of A549 cells. Key words paclitaxel; micelle; A549 cell; proliferation; cell cycle; apoptosis
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Polyesters
/
Polyethylene Glycols
/
Paclitaxel
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Cell Line, Tumor
/
Cell Proliferation
/
Bcl-2-Associated X Protein
Limits:
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2015
Type:
Article
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