Role of NF-κB in factor VIIa-induced proliferation and migration of colon cancer cell line SW620 cells / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 649-653, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-320113
ABSTRACT
<p><b>OBJECTIVE</b>To explore the roles of NF-κB in factor VIIa-induced proliferation and migration of a colon cancer cell line (SW620) in vitro and its possible mechanism.</p><p><b>METHODS</b>The expression levels of NF-κB (p65), inhibitory protein of NF-κB (IκB-α), caspase-7, interleukin 8 (IL-8) and tissue factor (TF) in SW620 cells treated with factor VIIa, PDTC (an inhibitor of NF-κB) and other factors were measured by Western-blotting and real-time PCR. Proliferation and migration of the cells were analyzed by flow cytometry and Transwell assay, respectively.</p><p><b>RESULTS</b>Factor VIIa down-regulated the IκB-α level in SW620 cells and increased the intranuclear level of NF-κB. Those effects of factor VIIa were blocked by anti-TF or anti-PAR2 antibodies. The effects of factor VIIa on proliferation and migration of SW620 cells, expression of IL-8, TF as well as caspase-7, were interfered by PDTC (the inhibitor of NF-κB).</p><p><b>CONCLUSIONS</b>TF/VIIa complex activates NF-κB pathway via PAR2, thereby up-regulates IL-8 and down-regulates caspase-7 expression in SW620 cells, finally promotes proliferation and migration of colon cancer cells. In addition, TF/VIIa/PAR2/NF-κB pathway also upregulates TF expression, thus to create a positive feedback loop of TF/VIIa/PAR2/NF-κB/TF.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Thiocarbamates
/
RNA, Messenger
/
Thromboplastin
/
Proline
/
Factor VIIa
/
Cell Movement
/
Interleukin-8
/
Colonic Neoplasms
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2011
Type:
Article
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