Effect of panax notoginseng saponin on procoagulant activity and differentiation induction in NB4 cells / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 63-66, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-320249
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Panax notoginseng saponin (PNS) on procoagulant activity (PCA) and differentiation induction in NB4 cells.</p><p><b>METHODS</b>After NB4 cells were treated with PNS, the recalcification time, PCA and TF-mRNA expression in NB4 cells were tested by RT-PCR. The inhibitory effect of PNS on NB4 cell proliferation was analysed by MTT method, NBT assay, cell morphological observation and flow cytometry.</p><p><b>RESULTS</b>(1) PNS of all concentrations could significantly prolong the recalcification time and lower the PCA level in NB4 cells in time-concentration-dependent manner. Simultaneously it down-regulated the expression of TF-mRNA. (2) PNS could partially inhibit the NB4 cell proliferation. (3) PNS could raise the NBT reducing capability of NB4 cells (P < 0.05). And morphological examination showed the differentiating tendency of monocyte and macrophage.</p><p><b>CONCLUSION</b>PNS could reduce the procoagulant activity and TF-mRNA expression in NB4 cells, and partially induce the differentiation of NB4 cells, therefore, it is hopeful to be a new anti-coagulant agent.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Saponins
/
Blood Coagulation Factors
/
Thromboplastin
/
Cysteine Endopeptidases
/
Tumor Cells, Cultured
/
Leukemia, Promyelocytic, Acute
/
Cell Division
/
Cell Transformation, Neoplastic
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2004
Type:
Article
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