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Effect of anti-fibrosis compound contained serum on procollagen Type I and IV, matrix metalloproteinase and its tissue inhibitor-1 gene expression in HSC-LI90 cell line / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 47-50, 2004.
Article in Chinese | WPRIM | ID: wpr-320253
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Anti-fibrosis Compound contained serum (AFCS) on procollagen type I and IV (ProC-I and ProC-IV), matrix metalloproteinase (MMP) and its tissue inhibitor (TIMP-1) gene expression in hepatic stellate cell line LI90 (HSC-LI90).</p><p><b>METHODS</b>AFCS was prepared by gastric infusing different dosage (0.5 g/kg, 2.0 g/kg and 4.0 g/kg) of Anti-fibrosis Compound Recipe to rats. After HSC-LI90 cells were exposed to AFCS for 48 hrs, levels of ProC-I, ProC-IV, gene expression of MMP-2, MMP membrane type 1 (MT1-MMP) and TIMP-1 in the cells were detected by Northern blot, and gelatinase activity of MMP-2 was measured by zymography.</p><p><b>RESULTS</b>AFCS of different concentrations could inhibit ProC-I and ProC-IV and TIMP-1 gene expression (P < 0.05 or P < 0.01), increase MT1-MMP gene expression (P < 0.01), but it showed no effect on gene expression and activity of MMP-2 (P > 0.05).</p><p><b>CONCLUSION</b>Anti-fibrosis Compound Recipe has anti-liver fibrosis action, its effects in inhibiting TIMP-1 gene expression of HSC-LI90 cells and promoting degradation of collagen might be one of the mechanisms of the action.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Blood / Drugs, Chinese Herbal / Gene Expression / Cell Line / Rats, Wistar / Tissue Inhibitor of Metalloproteinase-1 / Matrix Metalloproteinase 2 / Matrix Metalloproteinases Limits: Animals Language: Chinese Journal: Chinese Journal of Integrated Traditional and Western Medicine Year: 2004 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Blood / Drugs, Chinese Herbal / Gene Expression / Cell Line / Rats, Wistar / Tissue Inhibitor of Metalloproteinase-1 / Matrix Metalloproteinase 2 / Matrix Metalloproteinases Limits: Animals Language: Chinese Journal: Chinese Journal of Integrated Traditional and Western Medicine Year: 2004 Type: Article