A study of the technique of western blot for diagnosis of lyme disease caused by Borrelia afzelii in China / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 190-200, 2013.
Article
in English
| WPRIM
| ID: wpr-320351
ABSTRACT
<p><b>OBJECTIVE</b>To study the technique of Western blot for the diagnosis of Lyme disease caused by Borrelia afzelii in China and to establish the standard criteria by operational procedure.</p><p><b>METHODS</b>FP1, which is the representative strain of B. afzelii in China, was analyzed by SDS-PAGE, electro transfer and immunoblotting assays. The molecular weights of the protein bands of FP1 were analyzed by Gel-Pro analysis software. In a study using 451 serum samples (159 patients with Lyme disease and 292 controls), all observed bands were recorded. The accuracy of the WB as a diagnostic test was established by using the ROC curve and Youden index.</p><p><b>RESULTS</b>Criteria for a positive diagnosis of Lyme disease were established as at least one band of P83/100, P58, P39, OspB, OspA, P30, P28, OspC, P17, and P14 in the IgG test and at least one band of P83/100, P58, P39, OspA, P30, P28, OspC, P17, and P41 in the IgM test. For IgG criteria, the sensitivity, specificity and Youden index were 69.8%, 98.3%, and 0.681, respectively; for IgM criteria, the sensitivity, specificity and Youden index were 47%, 94.2%, and 0.412, respectively.</p><p><b>CONCLUSION</b>Establishment of WB criteria for B. afzelii is important in validating the diagnostic assays for Lyme disease in China.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virulence
/
Enzyme-Linked Immunosorbent Assay
/
Lyme Disease
/
Borrelia burgdorferi Group
/
China
/
Blotting, Western
/
Diagnosis
/
Electrophoresis, Polyacrylamide Gel
/
Methods
/
Microbiology
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2013
Type:
Article
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