Detrimental effect of electromagnetic pulse exposure on permeability of in vitro blood-brain-barrier model / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 128-137, 2013.
Article
in English
| WPRIM
| ID: wpr-320359
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of electromagnetic pulse (EMP) exposure on permeability of in vitro blood-brain-barrier (BBB) model.</p><p><b>METHODS</b>An in vitro BBB model, established by co-culturing brain microvascular endothelial cells (BMVEC) and astroglial cells (AC) isolated from rat brain, was exposed to EMP at 100 kV/m and 400 kV/m, respectively. Permeability of the model was assayed by measuring the transendothelial electrical resistance (TEER) and the horseradish peroxidase (HRP) transmission at different time points. Levels of BBB tight junction-related proteins were measured at 0, 1, 2, 4, 8, 12, 16, 20, 24 h after EMP exposure by Western blotting.</p><p><b>RESULTS</b>The TEER level was lower in BBB model group than in control group at 12 h after EMP, exposure which returned to its normal level at 24 h. The 24 h recovery process was triphasic and biphasic respectively after EMP exposure at 100 kV/m and 400 kV/m. Following exposure to 400 kV/m EMP, the HRP permeability increased at 1-12 h and returned to its normal level at 24 h. Western blotting showed that the claudin-5 and ZO-1 protein levels were changed after EMP exposure.</p><p><b>CONCLUSION</b>EMP exposure at 100 kV/m and 400 kV/m can increase the permeability of in vitro BBB model and BBB tight junction-related proteins such as ZO-1 and claudin-5 may change EMP-induced BBB permeability.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Radiation Effects
/
Capillary Permeability
/
Blood-Brain Barrier
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Electromagnetic Fields
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2013
Type:
Article
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