Differential responses to UVB irradiation in human keratinocytes and epidermoid carcinoma cells / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 583-589, 2012.
Article
in English
| WPRIM
| ID: wpr-320395
ABSTRACT
<p><b>OBJECTIVE</b>To examine UVB-induced responses in normal human keratinocytes (HaCaT) and epidermoid carcinoma cells (A431) at the cellular and molecular level, and investigated the protective effect of salidroside.</p><p><b>METHODS</b>Cells irradiated by UVB at various dosage and their viability was assessed by MTT assays, cell cycle was analysed by flow cytometry. The expression of NF-κB, BCL-2, and CDK6 after 50 J/m(2) UVB irradiation were detected by RT-PCR and western blotting.</p><p><b>RESULTS</b>Our results confirmed greater tolerance of A341 cells to UVB-induced damage such as cell viability and cell cycle arrest, which was accompanied by differential expression changes in NF-κB, BCL-2, and CDK6. UVB exposure resulted in HaCaT cells undergoing G(1)-S phase arrest. When treated with salidroside, HaCaT survival was significantly enhanced following exposure to UVB, suggesting great therapeutic potential for this compound.</p><p><b>CONCLUSION</b>Taken together, our study suggests that A431 respond differently to UVB than normal HaCaT cells, and supports a role for NF-κB, CDK6, and BCL-2 in UVB-induced cell G(1)-S phase arrest. Furthermore, salidroside can effectively protect HaCaT from UVB irradiation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phenols
/
Radiation Effects
/
Ultraviolet Rays
/
Carcinoma, Squamous Cell
/
Gene Expression Regulation, Neoplastic
/
Keratinocytes
/
Cell Survival
/
Apoptosis
/
Cell Line, Tumor
Limits:
Humans
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2012
Type:
Article
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