Expression of isoleucine zipper modified soluble CD40L in Pichia pastoris / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 844-847, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-320469
ABSTRACT
To obtain the expression of Isoleucine Zipper modified soluble CD40L (IZ-sCD40L) in Pichia pastoris, firstly, DNA fragment of IZ-sCD40L was obtained by PCR and over-lap PCR . Then the expression vector pPICZaA-IZ-sCD40L was constructed. Nucleotide sequencing analysis indicated that the DNA fragment of IZ-sCD40L was correctly inserted into the pPICZaA vector. Linearized pPICZ(alpha)A-IZ-sCD40L was introduced into Pichia pastoris GS115. Positive clone was selected by PCR and its phenotype was determined. The positive clone was introduced with methanol. The results of SDS-PAGE and Western blot showed that product was recombinant Isoleucine Zipper modified soluble CD40L fusion protein.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pichia
/
Plasmids
/
Recombination, Genetic
/
Recombinant Fusion Proteins
/
Angiotensin II
/
Polymerase Chain Reaction
/
Cloning, Molecular
/
CD40 Ligand
/
Genetics
/
Isoleucine
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2006
Type:
Article
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