Synthesis of 1,6-bis(L-alpha, beta-diaminopropionic acid) oxytocin / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 818-821, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-320476
ABSTRACT
A novel derivative of oxytocin containing nonprotein amino acid L-alpha, beta-diaminopropionic acid (L-Dap) was synthesized by 7+2 fragment combination in solution. N beta of all the amino acid necessary was protected by carbobenzoxy (Z) and N beta of L-Dap was protected by tert. -butoxycarbonyl (Boc) . The important intermediate, heptapeptide, was synthesized by the stepwise elongation method using carbobenzoxy amino acid p-nitrophenyl esters in solution. Azide synthesis was used to get the nonapeptide. Z group was removed by treatment with 5% Pd/C and Boc with CF3COOH. Eight new compounds incorporating L-Dap were obtained and confirmed by the amino acid analysis and mass spectral detection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxytocin
/
Chromatography, High Pressure Liquid
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2006
Type:
Article
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