MicroRNA-mRNA functional pairs for cisplatin resistance in ovarian cancer cells / 癌症
Chinese Journal of Cancer
;
(12): 285-294, 2014.
Article
in English
| WPRIM
| ID: wpr-320525
ABSTRACT
Ovarian cancer is the leading cause of death in women worldwide. Cisplatin is the core of first-line chemotherapy for patients with advanced ovarian cancer. Many patients eventually become resistant to cisplatin, diminishing its therapeutic effect. MicroRNAs (miRNAs) have critical functions in diverse biological processes. Using miRNA profiling and polymerase chain reaction validation, we identified a panel of differentially expressed miRNAs and their potential targets in cisplatin-resistant SKOV3/DDP ovarian cancer cells relative to cisplatin-sensitive SKOV3 parental cells. More specifically, our results revealed significant changes in the expression of 13 of 663 miRNAs analyzed, including 11 that were up-regulated and 2 that were down-regulated in SKOV3/DDP cells with or without cisplatin treatment compared with SKOV3 cells with or without cisplatin treatment. miRNA array and mRNA array data were further analyzed using Ingenuity Pathway Analysis software. Bioinformatics analysis suggests that the genes ANKRD17, SMC1A, SUMO1, GTF2H1, and TP73, which are involved in DNA damage signaling pathways, are potential targets of miRNAs in promoting cisplatin resistance. This study highlights candidate miRNA-mRNA interactions that may contribute to cisplatin resistance in ovarian cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovarian Neoplasms
/
Endopeptidases
/
Phosphoproteins
/
RNA, Messenger
/
Cysteine Endopeptidases
/
Nuclear Proteins
/
Chromosomal Proteins, Non-Histone
/
Signal Transduction
/
Down-Regulation
/
Up-Regulation
Type of study:
Prognostic study
Limits:
Female
/
Humans
Language:
English
Journal:
Chinese Journal of Cancer
Year:
2014
Type:
Article
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