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Effect of 1,25-(OH)2D3 supplementation during gestation and lactation on TGF-β1 and Smad3 expression in lungs of rat offspring with asthma / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics ; (12): 366-370, 2012.
Article in Chinese | WPRIM | ID: wpr-320644
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of 1,25-(OH)2D3 supplementation during gestation and lactation on TGF-β1 and Smad3 expression in lungs of rat offspring with asthma.</p><p><b>METHODS</b>Thirty-two female Wistar rats were randomly divided into four groups low-, medium- and high-dose 1,25-(OH)2D3 supplementation and control groups (n=8 each). From the 7th day of gestation, the three 1,25-(OH)2D3 supplementation groups were administered with 2,10 and 20 μg/mL of 1,25-(OH)2D3 respectively every other day until weaning (rat offspring 21 days old). The control group received normal saline instead. Then, bronchial asthma was induced in rat offspring from the 4 groups. The protein and mRNA expression of TGF-β1 and Smad3 in the lung tissue was measured by immunochemistry and RT-PCR.</p><p><b>RESULTS</b>Eosinophil cell infiltration and airway inflammation decreased in rat offspring from the low- and medium-dose 1,25-(OH)2D3 groups, but increased in rat offspring of the high-dose 1,25-(OH)2D3 group compared with the control group. Immunohistochemistry of lung tissues showed that the expression of TGF-β1 protein and pSmad3 decreased in rat offspring from the low- and medium-dose 1,25-(OH)2D3 groups (P<0.05), but increased significantly in rat offspring from the high-dose 1,25-(OH)2D3 group compared with the control group (P<0.05). PCR showed that the expression of TGF-β1 and Smad3 mRNA in the lung tissue decreased in rat offspring from the low- and medium-dose 1,25-(OH)2D3 groups (P<0.05), but increased significantly in rat offspring from the high-dose 1,25-(OH)2D3 group compared with the control group (P<0.05).</p><p><b>CONCLUSIONS</b>1,25-(OH)2D3 supplementation plays a role in regulating the immune system in asthmatic rats. Its mechanism may be associated with regulation of the expression of TGF-β/Smad signal pathway-related proteins through the vitamin D receptor signal pathway.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Physiology / Asthma / Lactation / RNA, Messenger / Signal Transduction / Rats, Wistar / Cholecalciferol / Dietary Supplements / Smad3 Protein Limits: Animals / Pregnancy Language: Chinese Journal: Chinese Journal of Contemporary Pediatrics Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Physiology / Asthma / Lactation / RNA, Messenger / Signal Transduction / Rats, Wistar / Cholecalciferol / Dietary Supplements / Smad3 Protein Limits: Animals / Pregnancy Language: Chinese Journal: Chinese Journal of Contemporary Pediatrics Year: 2012 Type: Article