Development and potential of a biomimetic chitosan/type II collagen scaffold for cartilage tissue engineering / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1436-1443, 2005.
Article
in English
| WPRIM
| ID: wpr-320752
ABSTRACT
<p><b>BACKGROUND</b>Damaged articular cartilage has very limited capacity for spontaneous healing. Tissue engineering provides a new hope for functional cartilage repair. Creation of an appropriate cell carrier is one of the critical steps for successful tissue engineering. With the supposition that a biomimetic construct might promise to generate better effects, we developed a novel composite scaffold and investigated its potential for cartilage tissue engineering.</p><p><b>METHODS</b>Chitosan of 88% deacetylation was prepared via a modified base reaction procedure. A freeze-drying process was employed to fabricate a three-dimensional composite scaffold consisting of chitosan and type II collagen. The scaffold was treated with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide. Ultrastructure and tensile strength of the matrix were carried out to assess its physico-chemical properties. After subcutaneous implantation in rabbits, its in vivo biocompatibility and degradability of the scaffold were determined. Its capacity to sustain chondrocyte growth and biosynthesis was evaluated through cell-scaffold co-culture in vitro.</p><p><b>RESULTS</b>The fabricated composite matrix was porous and sponge-like with interconnected pores measuring from 100-250 microm in diameter. After cross-linking, the scaffold displayed enhanced tensile strength. Subcutaneous implantation results indicated the composite matrix was biocompatible and biodegradable. In intro cell-scaffold culture showed the scaffold sustained chondrocyte proliferation and differentiation, and maintained the spheric chondrocytic phenotype. As indicated by immunohistochemical staining, the chondrocytes synthesized type II collagen.</p><p><b>CONCLUSIONS</b>Chitosan and type II collagen can be well blended and developed into a porous 3-D biomimetic matrix. Results of physico-chemical and biological tests suggest the composite matrix satisfies the constraints specified for a tissue-engineered construct and may be used as a chondrocyte carrier for cartilage tissue engineering.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tensile Strength
/
Biodegradation, Environmental
/
Immunohistochemistry
/
Cartilage
/
Chemistry
/
Coculture Techniques
/
Cell Biology
/
Collagen Type II
/
Tissue Engineering
/
Chitosan
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2005
Type:
Article
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