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HPLC-ELSD for determining contents of three triterpenoidal saponins in fruits of Buddleja lindleyana from different habitats / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 1218-1221, 2016.
Article in Chinese | WPRIM | ID: wpr-320875
ABSTRACT
To establish an HPLC-ELSD method for the quantification of triterpenoids in the fruits of Buddleja lindleyana. The RP-HPLC-ELSD method was used for the determination of triterpenoids in B. lindleyana fruits, which were collected from different habitats. The column used was a packed with 5 μm stationary phase Waters SunFireTM C₁₈ (4.6 mm×150 mm, 5 μm). The mobile phase consisted of Methanol-water(82∶18) at a flow rate of 1 mL•min⁻¹. Column temperature: 30 ℃. ELSD conditions: drift tube temperature: 106 ℃; carrier gas (nitrogen) flow rate: 1.5 L•min⁻¹; amplification factor: 1. The calibration curves showed good linear relationship on a range from 0.702 to 28.08 μg(r=0.999 2) for Clinoposaponin III, 0.390 to 15.60 μg(r=0.998 9) for Desrhamnoverbascosaponin and 0.192 to 7.68μg(r=0.999 0) for Mimengoside I. The average recovery rate(n=6) were 99.41%, 99.08% and 98.67% and it's RSD were 0.86%, 1.56% and 1.80%. This method can be used to determine the contents of triterpenoids in the fruits of Buddleja lindleyana for its simplicity, accurateness and reliability.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2016 Type: Article