Expression of imprinted genes related to Beckwith-Wiedemann syndrome in human oocytes and preimplantation embryos / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 265-267, 2005.
Article
in En
| WPRIM
| ID: wpr-321110
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of imprinted genes related to Beckwith-Wiedemann syndrome (BWS) in human oocytes and preimplantation embryos for understanding the relationship between assisted reproductive technology (ART) and BWS.</p><p><b>METHODS</b>Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.</p><p><b>RESULTS</b>Transcripts of P57KIP2 were detected in human oocytes and at all stages of preimplantation embryos. LIT1 was expressed only in stages of 8-cell and blastocyst. Transcripts of TSSC3 could not be detected in human oocytes and preimplantation embryos.</p><p><b>CONCLUSION</b>Transcripts of P57KIP2 and LIT1, imprinted genes related to BWS, were detected in human preimplantation development; ART might affect the epigenetics of imprinted genes in early embryogenesis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Oocytes
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Beckwith-Wiedemann Syndrome
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Blastocyst
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Nuclear Proteins
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Genomic Imprinting
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Reverse Transcriptase Polymerase Chain Reaction
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Gene Expression Profiling
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Potassium Channels, Voltage-Gated
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Cyclin-Dependent Kinase Inhibitor p57
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Genetics
Limits:
Female
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Humans
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Pregnancy
Language:
En
Journal:
Chinese Journal of Medical Genetics
Year:
2005
Type:
Article