Expression of mucin regulated by recombinant human interleukin-6 in HT-29 cell of colon cancer / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 899-902, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-321211
ABSTRACT
<p><b>OBJECTIVE</b>To study the regulation of the mucin protein in colon cancer cell line HT-29 by recombinant human interleukin-6(rIL-6) and to further elucidate the development of colon cancer.</p><p><b>METHODS</b>The HT-29 cells were treated with different concentrations of rIL-6(1, 2, 5, 10 and 20 μg/L), then flow cytometry and RT-PCR were used to detect the expression of mucin1 and mucin2. Transwell invasion assay was used to observe the effect of invasion capability of rIL-6 to HT-29 cells.</p><p><b>RESULTS</b>In colon cancer, the expression of mucin 1 could be promoted by rIL-6 with concentration above 2 μg/L, the expression rates were(12.5±1.6)%, (26.6±2.7)%, (33.9±2.8)% and (58.9±2.5)%, respectively, higher than (8.0±0.8)% in the negative controls (P<0.01), meanwhile, the expression of mucin 2 decreased by rIL-6 with concentration above 2 μg/L, the expression rates were(30.5±2.6)%, (17.0±2.7)%, (11.0±2.0)% and (5.3±1.8)%, respectively, lower than (41.6±3.6)% in negative control(P<0.01). With the increase in rIL-6 concentration, the invasion of HT-29 cells was enhanced.</p><p><b>CONCLUSIONS</b>In colon cancer, the expression of mucin1 can be promoted by rIL-6, while the expression of mucin2 can be inhibited. IL-6 is a promoting effect factor in colon cancer invasion and metastasis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Recombinant Proteins
/
Interleukin-6
/
Colonic Neoplasms
/
Mucin-1
/
HT29 Cells
/
Mucin-2
/
Flow Cytometry
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2011
Type:
Article
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