Tissue inhibitor of metalloproteinase-1 counteracts glucolipotoxicity in the pancreatic β-cell line INS-1 / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 258-261, 2011.
Article
in English
| WPRIM
| ID: wpr-321458
ABSTRACT
<p><b>BACKGROUND</b>Glucolipotoxicity might play an important role in the β cell decompensation stage during the development of obesity-associated type 2 diabetes. Tissue inhibitor of metalloproteinase-1 (TIMP-1) inhibits matrix metalloproteinase (MMP) activity and regulates proliferation and apoptosis of a variety of cell types, including pancreatic β-cells. In the present study, we investigated whether TIMP-1 counteracts glucolipotoxicity in the pancreatic β-cell line INS-1.</p><p><b>METHODS</b>INS-1 cells were incubated in normal or high glucose, with or without palmitate (0.4 mmol/L), in the presence of TIMP-1 or MMP inhibitor GM60001. In some experiments, cells were pretreated with phosphatidylinositol-3 (PI-3) kinase inhibitor, LY294002 or wortmannin. The amount of dead INS-1 cells was determined by HO342 and propidium iodide staining. Akt phosphorylation was evaluated by Western blotting analysis to investigate a possible mechanism of TIMP-1's action.</p><p><b>RESULTS</b>TIMP-1 protected INS-1 cells from glucolipotoxicity independent of MMP inhibition. TIMP-1 stimulated Akt phosphorylation. Inhibition of the PI-3 kinase pathway abolished the survival effect of TIMP-1.</p><p><b>CONCLUSION</b>TIMP-1 may counteract glucolipotoxicity induced β-cell death via a PI-3 kinase pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Palmitates
/
Pharmacology
/
Phosphorylation
/
Signal Transduction
/
Cell Line
/
Tissue Inhibitor of Metalloproteinase-1
/
Phosphatidylinositol 3-Kinases
/
Insulin-Secreting Cells
/
Proto-Oncogene Proteins c-akt
/
Glucose
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2011
Type:
Article
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