p53 in fibroblast-like synoviocytes can regulate T helper cell functions in patients with active rheumatoid arthritis / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 364-368, 2011.
Article
in English
| WPRIM
| ID: wpr-321501
ABSTRACT
<p><b>BACKGROUND</b>p53 is a tumor suppressor and plays a key role in regulating cell hyperplasia, repairing DNA and inducing apoptosis. This study was to investigate p53 expression in fibroblast-like synoviocytes (FLS) and its effect on CD4(+) T lymphocytes from patients with active rheumatoid arthritis (RA).</p><p><b>METHODS</b>Human FLS were transfected with p53 siRNA and cocultured with CD4(+) T lymphocytes from patients with active RA. The expressions of osteoprotegerin and interleukin (IL)-6 were detected in p53 siRNA and scramble siRNA-transfected FLS. In addition, protein levels of interferon (IFN)-γ, IL-17, IL-4 and CD25 as well as mRNAs of IFN-γ, retinoic acid-related orphan receptor (ROR)-γt, IL-17 and Foxp3 in cocultured CD4(+) T lymphocytes were also measured.</p><p><b>RESULTS</b>IL-6 decreased in p53-knockdown FLS while osteoprotegerin expression was not altered. FLS with p53 deletion significantly increased the production of IL-17 and IFN-γ by CD4(+) T cells and upregulated Foxp3 mRNA expression without effects on the proportion of CD4(+)CD25(high) T lymphocytes.</p><p><b>CONCLUSION</b>p53 in FLS might regulate Th1 and Th17 functions in patients with RA and participate in the pathogenesis of RA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arthritis, Rheumatoid
/
Synovial Membrane
/
Enzyme-Linked Immunosorbent Assay
/
CD4-Positive T-Lymphocytes
/
Cells, Cultured
/
Tumor Suppressor Protein p53
/
Interleukin-6
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Biology
/
Allergy and Immunology
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2011
Type:
Article
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