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Kiss-1 gene expression after radiation and its association with proliferation and apoptosis in colorectal cancer cells / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery ; (12): 508-511, 2012.
Article in Chinese | WPRIM | ID: wpr-321591
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the change of expression level of metastasis suppressor gene Kiss-1 in the colorectal cancer cell line SW480 after radiation, and to determine its association with the proliferation and apoptosis of SW480 cells.</p><p><b>METHODS</b>SW480 cells were divided into control group (0 Gy) and study groups (2, 4, 6, 8 Gy). Cells in the study groups were irradiated by 6-MV X-ray radiation for 48 hours. Immunohistochemistry and real-time PCR methods were used to investigate the influence of radiation on Kiss-1 gene expression of SW480. Colony formation assay was used to detect the proliferation of SW480. Flow cytometry-Annexin- V/PI assay was used to observe the change of the apoptosis rate.</p><p><b>RESULTS</b>Compared with the control group, Kiss-1 protein expression increased after radiation of 6, 8 Gy (P<0.05), but no significant changes were observed after radiation of 2, 4 Gy(P>0.05). Kiss-1 gene mRNA level increased after radiation of 2, 4, 6 Gy, while no obvious change was observed for 8 Gy radiation. The apoptosis rates increased for 4, 6, 8 Gy radiation(P<0.05), however, there was no significant difference for 2 Gy radiation (P<0.05).</p><p><b>CONCLUSION</b>Radiation may increase Kiss-1 gene expression in SW480 cells, which results in decreases proliferation and increases apoptosis in residual surviving cells.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Radiation Effects / X-Rays / RNA, Messenger / Colorectal Neoplasms / Apoptosis / Cell Line, Tumor / Cell Proliferation / Kisspeptins / Genetics Limits: Humans Language: Chinese Journal: Chinese Journal of Gastrointestinal Surgery Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Radiation Effects / X-Rays / RNA, Messenger / Colorectal Neoplasms / Apoptosis / Cell Line, Tumor / Cell Proliferation / Kisspeptins / Genetics Limits: Humans Language: Chinese Journal: Chinese Journal of Gastrointestinal Surgery Year: 2012 Type: Article