Determination of plasma escitalopram with liquid chromatography-mass spectrometry / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To establish an liquid chromatography-mass spectrometry (HPLC-MS/MS)-based method for determining escitalopram in human plasma.</p><p><b>METHODS</b>A liquid-liquid ether extraction approach was adopted using dextromethorphan as the internal standard. The Agilent ZORBAX SB-C18 (3.5 microm,2.1 x 150 mm) was used as the analytical column with acetonitrile-NH4AC buffer (7030, V/V) as the mobile phase at the flow rate of 0.3 ml/min. The sample was ionized by electrospray ionization source in the triple quadruple tandem mass spectrometer, and the plasma escitalopram determined with a multiple reaction monitoring mode of m/z325.4-->109.4.</p><p><b>RESULTS</b>The linear range was 0.0866-64.13 microg/L (r=0.9965) for escitalopram in human plasma, with the absolute recovery between 64.98% and 78.72% and the within-day and between-day deviations less than 10%.</p><p><b>CONCLUSION</b>The method is sensitive, accurate, rapid, specific and well applicable for clinical pharmacokinetics study of escitalopram.</p>