Extraction and two-dimensional liquid chromatographic fractionation of plasma membrane proteome of mouse liver cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1947-1949, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-321785
ABSTRACT
<p><b>OBJECTIVE</b>To extract the plasma membrane proteins from mouse liver cells and investigate the approach for fractionating the protein mixtures by two-dimensional liquid chromatography.</p><p><b>METHODS</b>The plasma membrane of the liver cells from 10 mice was extracted by differential centrifugation and sucrose density-gradient centrifugation. The plasma membrane proteins were exchanged with the start buffer and separated by chromatofocusing in the first-dimensional fractionation. The final results were transformed into UV/pI maps using ProteoVue software.</p><p><b>RESULTS</b>We successfully extracted the plasma membrane proteins from mouse liver cells. Sixteen fractions between pH 8.5-4.0 were recovered in the first-dimensional chromatofocusing followed by 2D- chromatographic fractionation, and the results were displayed as UV/pI maps.</p><p><b>CONCLUSION</b>This approach for fractionating the mouse liver cell plasma membrane protein study provides the foundation for further studies on the functions of plasma membrane proteins and differential proteome of diseases.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Membrane
/
Chromatography, Liquid
/
Proteome
/
Cell Biology
/
Proteomics
/
Chemical Fractionation
/
Liver
/
Metabolism
/
Methods
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
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