Transfection with human adenovirus type 5 early-region 1A protects the primary human fibroblasts from ras-induced premature senescence in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1820-1823, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-321808
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of human adenovirus type 5 (Ad5) early-region 1A (E1A) in premature senescence of human fibroblasts induced by Ras activation.</p><p><b>METHODS</b>Human fibroblasts were cotransduced with E1A, E1A1-143, or their vector (BP) and Ha-RasV12 or its vector (WH). The growth curves and percentages of the apoptotic cells were determined.</p><p><b>RESULTS</b>Expression of E1A or Ha-RasV12 in human fibroblasts significantly inhibited the cell growth. Transduction of Ha-RasV12 along with E1A or E1A 1-143 into human fibroblast cells resulted in active and rapid cell proliferation.</p><p><b>CONCLUSION</b>E1A can rescue human fibroblasts from Ras-induced premature senescence, and the senescence bypassing activity of E1A resides in its NH2 terminus.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Skin
/
Transfection
/
Cell Transformation, Neoplastic
/
Cells, Cultured
/
Genes, ras
/
Cellular Senescence
/
Apoptosis
/
Adenovirus E1A Proteins
/
Cell Biology
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
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