Effect of homocysteine on gluconeogenesis in mice / 南方医科大学学报
Journal of Southern Medical University
;
(12): 507-510, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-322013
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expressions of glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK) in the liver of mice with hyperhomocysteinemia (HHcy) and explore the mechanism of gluconeogenesis induced by homocysteine.</p><p><b>METHODS</b>Fifty mice were randomly divided into normal control group (n=25) and HHcy group (n=25) and fed with normal food and food supplemented with 1.5% methionine, respectively. After 3 months of feeding, the fasting blood glucose and insulin levels were determined, and HOMA insulin resistance index (HOMA-IR) was calculated. The expressions of G6Pase and PEPCK in the liver of mice were detected using RT-PCR and Western blotting.</p><p><b>RESULTS</b>The fasting blood glucose and insulin levels and HOMA-IR were significantly higher in HHcy group than in the control group (P<0.05). RT-PCR and Western blotting showed that the hepatic expressions of G6Pase and PEPCK mRNA and proteins increased significantly in HHcy group compared with those in the control group (P<0.05).</p><p><b>CONCLUSION</b>Homocysteine promotes gluconeogenesis to enhance glucose output and contribute to the occurrence of insulin resistance.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Insulin Resistance
/
Glucose-6-Phosphatase
/
Phosphoenolpyruvate Carboxykinase (ATP)
/
Hyperhomocysteinemia
/
Gluconeogenesis
/
Homocysteine
/
Liver
/
Metabolism
/
Mice, Inbred Strains
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article
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