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Screening of hepatocyte proteins interacting with hepatitis B virus X protein using CytoTrap yeast two-hybrid technique / 南方医科大学学报
Journal of Southern Medical University ; (12): 486-490, 2013.
Article in Chinese | WPRIM | ID: wpr-322017
ABSTRACT
<p><b>OBJECTIVE</b>To screen the hepatocyte proteins that interact with hepatitis B virus X protein (HBx).</p><p><b>METHODS</b>The recombinant plasmid pSos-HBx was constructed by inserting Sos-HBx fragment into the bait vector, and after sequence verification the plasmid was transformed into competent yeast cells. The expression and self-activation of Sos-HBx protein was detected in the yeast cells. The hepatocyte proteins interacting with the bait protein was screened with CytoTrap yeast two-hybrid technique.</p><p><b>RESULTS</b>The reconstructed plasmid harboring HBx gene expressed Sos-HBx protein in the yeast cells without self-activation of the protein. CytoTrap yeast two-hybrid system identified 6 hepatocyte proteins that interacted with HBx, including fibronectin 1, translationally controlled tumor protein, IQ motif and WD repeats 1, follistatin, orosomucoid 1, and disulfide isomerase family A member 3.</p><p><b>CONCLUSION</b>Six HBx-binding hepatocyte proteins have been identified using the CytoTrap yeast two-hybrid system, which provides clues for further investigation of the role of HBx protein in hepatitis and liver cancer.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Proteins / Trans-Activators / Two-Hybrid System Techniques / Hepatocytes / Protein Interaction Domains and Motifs / Genetic Vectors / Metabolism Type of study: Diagnostic study / Prognostic study / Screening study Limits: Humans Language: Chinese Journal: Journal of Southern Medical University Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Proteins / Trans-Activators / Two-Hybrid System Techniques / Hepatocytes / Protein Interaction Domains and Motifs / Genetic Vectors / Metabolism Type of study: Diagnostic study / Prognostic study / Screening study Limits: Humans Language: Chinese Journal: Journal of Southern Medical University Year: 2013 Type: Article