Effects of HSP90 inhibitor 17-AAG on cell cycle and apoptosis of human gastric cancer cell lines SGC-7901 / 南方医科大学学报
Journal of Southern Medical University
;
(12): 271-275, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-322066
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of the HSP90 inhibitor, 17-allylamino-17-demethoxygeldanamycin (17-AAG), on cell proliferation and apoptosis of human cancer SGC-7901 cells and explore the mechanisms.</p><p><b>METHODS</b>The inhibitory effect of 17-AAG on the proliferation and morphology of SGC-7901 cells was assessed with MTT assay and DNA-PI staining, respectively. Flow cytometry was employed to analyze the changes in cell cycle and apoptosis of the cells following 17-AAG exposure. The cellular expression of Fas protein was detected by immunohistochemistry.</p><p><b>RESULTS</b>17-AAG significantly suppressed the proliferation of SGC-7901 cells in a time- and dose-dependent manner. After treatment with 17-AAG for 48 h, SGC-7901 cells showed cell cycle arrested at G(2)/M stage, and the cell apoptosis rate increased with the 17-AAG concentration. The expression of Fas protein in the cytoplasm of SGC-7901 cells increased gradually with the increase of 17-AAG concentration.</p><p><b>CONCLUSION</b>17-AAG can induce apoptosis, alters the cell cycle distribution and up-regulates the expression of Fas protein in SGC-7901 cells to suppress the cell proliferation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Stomach Neoplasms
/
Cell Cycle
/
Benzoquinones
/
Apoptosis
/
HSP90 Heat-Shock Proteins
/
Fas Receptor
/
Cell Line, Tumor
/
Lactams, Macrocyclic
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article
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