The intercellular communication condition of alveolar bone with traumatic occlusion at early stage in rats / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the intercellular communication of alveolar bone during traumatic occlusion at early stage in rats.</p><p><b>METHODS</b>The occlusal surface of the upper left first molar of rat was raised by placing a stainless steel wire to induce occlusal trauma in the lower left first molar. After 24 hours, the alveolar bone tissues of the lower jaws first molars at the both sides were taken out under anesthesia The various 27 000 genes were identified with genome-wide microarray, and further were investigated with reverse transcription-polymerase chain reaction (RT-PCR) and Pathway analysis.</p><p><b>RESULTS</b>Total 586 gene were found to be changed, 106 different signal pathways got involved with Pathway analysis, including cell adhesion molecules(CAMS), adhesions junction, gap junction, focal adhesion and tight junction, and the cytokines associated with bone metabolism in above 5 signal pathways were all down-regulated.</p><p><b>CONCLUSION</b>At the early phase of the occlusal trauma, intercellular communication in rat's alveolar bone were inhibited.</p>