Effects of intracellular osmolality changes on the voltage-gated sodium channels currents of trigeminal ganglion neuron / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 338-342, 2012.
Article
in Zh
| WPRIM
| ID: wpr-322388
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the biomechanical effects of intracellular changes on the voltage-gated sodium channels (VGSCs) on trigeminal ganglion neuron (TRGN).</p><p><b>METHODS</b>TRGN cells were acutely isolated from the neonatal SD rats. The voltage-dependent currents of the VGSCs on these neurons were elicited and analyzed by whole-cell patch-clamp recordings and the intracellular anisotonicity stimuli was established by adjusting the content of pipette solution. The effects of hypo-(260 mOsm) and hypertonic (350 mOsm) osmolarity on the activation and inactivation kinetics of VGSCs on TRGN were evaluated, compared with the normal intracellular environment.</p><p><b>RESULTS</b>The results demonstrated that intracellular hypotonic stimuli could influence both the activation and inactivation characteristics of VGSCs currents, including the membrane potential at half inactivation (V0.5) of the G-V and inactivation curves had obvious statistics significance (P<0.05) between hypotonicity (260mOsm) and isotonicity (306mOsm). However, only inactivation properties changed under intracellular hypertonic effects, including inactivation rate and k value.</p><p><b>CONCLUSION</b>It suggests that the kinetics of VGSCs on TRGN can be modulated both by intracellular hypo- and hypertonic with different characteristics.</p>
Full text:
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Index:
WPRIM
Main subject:
Osmolar Concentration
/
Sodium Channels
/
Cells, Cultured
/
Trigeminal Ganglion
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Rats, Sprague-Dawley
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Patch-Clamp Techniques
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Membrane Potentials
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Neurons
Limits:
Animals
Language:
Zh
Journal:
West China Journal of Stomatology
Year:
2012
Type:
Article