Effects of newborn bull serum and vitamins on cryopreservation of mouse seminiferous epithelial cells / 中华男科学杂志
National Journal of Andrology
;
(12): 244-246, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-322604
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the effects of newborn bull serum(NBS), vitamin C and vitamin E on cryopreservation of mouse seminiferous epithelial cells.</p><p><b>METHODS</b>The seminiferous epithelial cells from 7-day-old mice were cryopreserved in different freezing solutions. The cell recoveries were examined by Trypan blue exclusive staining after thawing. The freezing solutions composed of DMEM, 10% dimethylsulphoxide(DMSO), and 0, 5%, 10%, or 20% NBS, respectively, or composed of DMEM, 10% DMSO, 10% NBS, and 150 micrograms/ml vitamin C or 50 micrograms/ml vitamin E, respectively.</p><p><b>RESULTS</b>The cell recoveries in freezing solution containing 0, 5%, 10%, or 20% NBS were 83.4%, 84.7%, 85.7% and 83.6%, respectively. There were no significant differences between them. The cell recoveries in freezing solution containing vitamin C or vitamin E were 88.0% and 82.9%, respectively. There was no significant differences compared with that in freezing solution containing 10% DMSO and 10% NBS.</p><p><b>CONCLUSIONS</b>NBS, vitamin C and vitamin E have no significant protecting effects on mouse seminiferous epithelial cells, and can not significantly improve the cell recoveries.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Ascorbic Acid
/
Seminiferous Epithelium
/
Vitamin K
/
Cryopreservation
/
Cell Biology
/
Epithelial Cells
/
Fetal Blood
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2002
Type:
Article
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