Determination of ascitic bacterial 16S rRNA gene in the rapid diagnosis of spontaneous bacterial peritonitis / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 557-560, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-322733
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the value of ascitic bacterial 16S rRNA gene determination in the rapid diagnosis of spontaneous bacterial peritonitis (SBP).</p><p><b>METHODS</b>16S rRNA gene from bacterial DNA in ascites was determined by quantitative fluorescent polymerase chain reaction (PCR) in 76 patients with suspected SBP and 6 patients with non-infectious ascites. The results were compared with those obtained from bacterial culture.</p><p><b>RESULTS</b>The positive rate of SBP was 22.4% among patients detected with ascitic bacterial 16S rRNA gene determination-based quantitative fluorescent PCR, which was significantly higher than that (7.9%) in patients only received bacterial culture (P<0.05). In addition,in 6 patients with non-infectious ascites,both the 16S rRNA gene determination-based quantitative fluorescent PCR and bacterial culture showed negative results.</p><p><b>CONCLUSIONS</b>16S rRNA gene determination-based quantitative fluorescent PCR can be an effective tool for the rapid diagnosis of SBP. It is more sensitive than the bacterial culture.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peritonitis
/
Bacterial Infections
/
Ascitic Fluid
/
DNA, Bacterial
/
RNA, Ribosomal, 16S
/
Diagnosis
/
Microbiology
Type of study:
Diagnostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2010
Type:
Article
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