Study on molecular epidemiology of people infected with human immunodeficiency virus-1 in Hubei province / 中华流行病学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the distribution of human immunodeficiency virus-1 (HIV-1) genotypes in Hubei province.</p><p><b>METHODS</b>Epidemiological survey was carried out to HIV-1 carriers who were identified in Hubei province. HIV-1 env V3-V4, gag P17/24 and the first exon of tat region were amplified by nested-polymerase chain reaction(nPCR) .The sequences were determined, and phylogenetic analyses were then performed.</p><p><b>RESULTS</b>4 HIV-1 strains or circulating recombinant forms (CRFs) were identified in Hubei province with subtype B' the predominant which covered 5 kinds of populations including former blood donors, blood receivers, spouses of the infected people, sex workers and their clients, homosexuals, mainly distributed in the areas with many former blood donors. CRF08-BC and CRF01-AE were found distributed in economically more developed cities or southern area of the province, and the major transmission routes was through sexual contact. Only 1 patient, an injecting drug user, was identified having subtype C.</p><p><b>CONCLUSION</b>Subtype B' was the main epidemic subtypes in Hubei province while CRF08-BC, CRF01-AE and subtype C were also circulating in the province, indicating the transmission of the disease might to become more complex.</p>