Genetic diversity in 19 Chinese populations of Oncomelania hupensis (Gastropoda: Rissooidea) detected by simple sequence repeat-anchored polymerase chain reaction amplification / 中华流行病学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the genetic diversity among populations of Oncomelania hupensis.</p><p><b>METHODS</b>Simple sequence repeat-anchored PCR method was used to amplify the genomic DNA of nineteen snail populations from 8 provinces, and the genetic diversities among these snail populations were analyzed.</p><p><b>RESULTS</b>The genetic distance D among nineteen snail populations ranged from 0 to 0.73 with average genetic distance as D 0.22 +/- 0.013. All the 19 snail populations were clustered into four groups one group included 16 snail populations distributed throughout the Yangtze River drainage system below the Three Gorges of the river (Hunan, Hubei, Jiangxi, Anhui, and Jiangsu provinces); Snail populations from Yizhou city, Guangxi province, Fuqing city from Fujian province, and Dali city from Yunnan province belonged to an independent group respectively.</p><p><b>CONCLUSION</b>The distribution of Oncomelania hupensis in mainland China could be divided into four subspecies, i.e. Oncomelania hupensis hupensis (O. h. hupensis ), O. h. robertsoni, O. h. tangi , and O. h. guangxiensis.</p>