Simultaneous determination of 11 opiates in hair by liquid chromatography-tandem mass spectrometry / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1501-1506, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-323094
ABSTRACT
The paper reports the establishment of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) for simultaneous analysis of 11 opiates in hair samples, and the study of presence of opiates in the hair of active heroin addicts. About 20 mg of decontaminated and pulverized hair sample was hydrolyzed with buffer solution for 30 min, in the presence of morphine-d3 and acetylmorphine-d6 used as internal standards, and then extracted with the mixture of dichlormethane and isopropanol, separated by the Allure PFP propyl column with a mobile phase consisting of acetonitrile and 20 mmol L(-1) ammonium acetate buffer, and then analyzed by LC-MS/MS. Multiple reaction monitoring (MRM) mode was used to analyze 11 opiates. Eleven opiates showed a fairly good linearity over the corresponding range (r > 0.996 0). The detection limits were less than 0.05 ng mg(-1). The recoveries were between 47.2% and 110%, and the deviations of intra- and inter-day precision were less than 14%. Heroin, acetylmorphine, morphine, codeine, acetylcodeine and hydrocodone were detected in hair samples of 21 herion addicts. The developed method shows high sensitivity and selectivity, and is suitable for the simultaneous analysis of 11 opiates in hair samples and identify legal and illegal use of opiates.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Substance Abuse Detection
/
Chemistry
/
Chromatography, Liquid
/
Sensitivity and Specificity
/
Codeine
/
Heroin
/
Tandem Mass Spectrometry
/
Limit of Detection
/
Hair
/
Hydrocodone
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2011
Type:
Article
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