Culture and identification of human adult Leydig cells / 中华男科学杂志
National Journal of Andrology
;
(12): 356-358, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-323360
ABSTRACT
<p><b>OBJECTIVE</b>To obtain a simple and effective method to isolate and purify adult Leydig cells.</p><p><b>METHODS</b>The testes of human adults were digested and then the density gradient centrifugation of the cells was performed with four different Percoll concentrations (60%, 34%, 26%, 21%) to isolate Leydig cells, whose characteristics were identified by cytological observation staining, 3beta-HSD staining and detection of hCG and testosterone secretion.</p><p><b>RESULTS</b>High-concentration (> 90%) purified Leydig cells were acquired, and many identification experiments demonstrated the adequate testosterone secretory function of the isolated and purified Leydig cells.</p><p><b>CONCLUSION</b>This method is easy and efficient for the isolation and purification of adult Leydig cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Testosterone
/
Cell Separation
/
Cells, Cultured
/
Povidone
/
Silicon Dioxide
/
Bodily Secretions
/
Cell Biology
/
Chorionic Gonadotropin
/
Leydig Cells
Type of study:
Prognostic study
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2005
Type:
Article
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