Effects of pentachlorophenol on rat sertoli cells / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To detect the toxic effects of pentachlorophenol (PCP) on cultured rat Sertoli cells.</p><p><b>METHODS</b>The viability of Sertoli cells was detected and morphological examination was performed, followed by flow cytometric assay to evaluate the toxic effect of PCP on rat Sertoli cells.</p><p><b>RESULTS</b>MTT assay showed that PCP induced a concentration- and time-dependent decrease in Sertoli cell viability. Flow cytometric assay revealed that the number of dead Sertoli cells grew along with increased exposure to PCP.</p><p><b>CONCLUSION</b>PCP, with obvious cytotoxic effects, can cause necrosis of Sertoli cells in vitro.</p>