Impact of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2 / 中华男科学杂志
National Journal of Andrology
;
(12): 256-260, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-323383
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of PC-1 gene knockdown on the biological action of prostate cancer cell line C4-2.</p><p><b>METHODS</b>Recombinant plasmids of expressing short hairpin RNA targeting PC-1 mRNA were constructed using DNA recombinant technology and transfected into C4-2 cells via liposome. The positive cell clones were selected by G418. The expression of PC-1 gene was analyzed by RT-PCR and Western blotting technology. MTT and soft agar cloning formation were applied to observe the changes of the growth rate and independent anchor ability of C4-2 cells.</p><p><b>RESULTS</b>PC-1 RNA interference severely affected the expression of PC-1 gene and reduced the growth and colony formation ability of C4-2 cells.</p><p><b>CONCLUSION</b>RNA interference-mediated PC-1 gene knockdown can decrease the growth and cloning formation ability of C4-2 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Prostatic Neoplasms
/
Pyrophosphatases
/
RNA, Messenger
/
Transfection
/
Down-Regulation
/
Gene Expression
/
Phosphoric Diester Hydrolases
/
RNA Interference
/
Cell Line, Tumor
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2005
Type:
Article
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