Preparation and biocompatibility evaluation of osteochondral composite scaffold / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To prepare osteochondral composite scaffold and study its biocompatibility in vitro.</p><p><b>METHODS</b>The composite material of nano-HAP/collagen I was prepared, and the osteochondral scaffold was manufactured by combining nano-HAP, collagen I, and PLGA as the bone section and sodium hyaluronate and PLGA as the chondral section. The diameter, chemical composition and crystallinity of the nano-HAP/collagen I composite particles were assessed with TEM, FTIR and XRD, and the biocompatibility and cytotoxicity of the scaffold were evaluated using MTT assay by co-culturing bone marrow stem cells and the scaffold.</p><p><b>RESULTS AND CONCLUSION</b>The osteochondral composite scaffold has good microstructure without obvious cytotoxicity, possesses good biocompatibility with bone marrow stem cells and is suitable as an osteochondral scaffold material.</p>