Variations of the amount of sialic acids on hepatocellular carcinoma cell membrane / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2323-2326, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-323671
ABSTRACT
<p><b>OBJECTIVE</b>To observe the change in the amount of sialic acids on hepatocellular carcinoma (HCC) cell membrane.</p><p><b>METHODS</b>Surgical specimens of HCC and liver cirrhosis tissues were obtained from 28 patients to prepare carcinoma cell and hepatocyte suspensions by collagenase digestion. For assay of α2, 3 and α2, 6-sialic acids, the cells were suspended in the staining buffer containing either fluorescein isothiocyanate-Maackia amurensis lectin (FITC-MAL) or fluorescein isothiocyanate-Sambucus nigra bark lectin (FITC-SNA) and incubated for 1 h, respectively. Flow cytometric analysis was carried out to measure the mean fluorescence intensity (MFI) on the cell surface.</p><p><b>RESULTS</b>In both FITC-MAL- and FITC-SNA-incubated HCC cells, the MFI on the cell surface was greater than that of the hepatocytes.</p><p><b>CONCLUSION</b>Both of α2, 3 and α2, 6- sialic acids increases significantly on the hepatocyte membrane after the carcinomatous change.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Sialic Acids
/
Cell Membrane
/
Carcinoma, Hepatocellular
/
Liver Cirrhosis
/
Liver Neoplasms
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article
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