Telomerase SiRNA inhibits KB cell growth in human oral squamous cell carcinoma / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 376-378, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-324142
ABSTRACT
<p><b>OBJECTIVE</b>To test the telomerase SiRNA on telomerase mRNA and on KB cell growth of oral squamous cell carcinoma.</p><p><b>METHODS</b>We synthesized 21-nucleotide SiRNA duplexes with symmetric 2-nucleotide 3' overhangs corresponding to the target sequence (2 657 approximately 2 675 nucleotide downstream of the start codon) of telomerase mRNA. Telomerase activity, cell proliferation, cell cycle and apoptosis were measured after transfection.</p><p><b>RESULTS</b>Twenty one-nucleotide small interfering RNA (SiRNA) duplexes specifically suppressed expression of endogenous telomerase mRNA in human oral squamous carcinoma KB cells. This inhibitory effect lasted only for about 48 h after transfection. Telomerase activity reduction corresponded to the mRNA suppression. Cell proliferation decreased by 30% at 48 h after transfection and lasted for 120 h after treatment. This inhibitory effect resulted from the block of G(1) to S transition. Apoptosis was not involved in this process.</p><p><b>CONCLUSIONS</b>SiRNA is a powerful tool for studying gene function and can be used as gene-specific therapeutics.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
RNA, Messenger
/
Mouth Neoplasms
/
Tumor Cells, Cultured
/
KB Cells
/
Carcinoma, Squamous Cell
/
Apoptosis
/
Telomerase
/
RNA, Small Interfering
/
Cell Proliferation
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2004
Type:
Article
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