Determination of methyl isocyanate (MIC) in air of workplaces by HPLC / 中华劳动卫生职业病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish the method of detecting the concentrations of methyl isocyanate (MIC) in air of workplaces with high performance liquid chromatographic (HPLC).</p><p><b>METHODS</b>Samples are collected by XAD-7 tubes coated with 1-(2-pyridyl) piperazine (1-2PP). Samples are desorbed with acetonitrile (ACN) and analyzed by high performance liquid chromatography (HPLC) using a fluorescence detector.</p><p><b>RESULTS</b>There was a linear relationship within the range of 0.01 ∼ 10 µg/ml, and the detection limit was 5.3×10(-4) µg/ml. The minimum detectable concentration was 0.01 µg/ml, and the lowest detected concentration was 3.3×10(-3) mg/m(3). The relative standard deviation was 2.2% ∼ 5.3%. The average desorption efficiency was 90% and the sampling efficiencies were 100%. The samples could be stored for 10 days in cold storage condition.</p><p><b>CONCLUSION</b>The present method could meet with the requirements of Guide for establishing occupational health standards-Part4 Determination methods of air chemicals in workplace and be feasible for determination of MIC in workplace air.</p>