The optimization of 2-thiothiazolidine-4-carboxylic acid back extraction in urine and determination by HPLC / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 473-474, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-324218
ABSTRACT
<p><b>OBJECTIVE</b>Establishment of determination method of 2-thiothiazolidine-4-carboxylic acid (TTCA) in urine with HPLC.</p><p><b>METHODS</b>A volume of 0.5 ml hydrochloric acid (2 mol/L) and 0.5 ml pure water was added into 1 ml urine, and then extracted by 4 ml of diethyl ether by shaking for 2 min. Remove the water phase in a tube with plug and extract again, mix the two extraction diethyl ether together, take 4 ml by adding 2 ml borax-monopotassium phosphate buffer and shaking for 2 min to extract, then take the water phase to detect. A C(18) column and UV detector were used for separating and detecting. The wavelength was 273 nm, the flow rate was 1.0 ml/min, and the injection volume was 20 µl.</p><p><b>RESULTS</b>TTCA has a good linearity (r = 0.9995) over the concentration of1 1 ∼ 10 µg and the minimum detectable concentration of TTCA in urine was 0.1 µg/ml. The within-day precision (RSD) were 8.4%, 3.0% and 1.7%, the between-day precision (RSD) were 11%, 3.8%, 1.9%, respectively. The extraction recovery were between 80% ∼ 102%.</p><p><b>CONCLUSION</b>The method was accurate and sensitive to detect TTCA in urine.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Urine
/
Carbon Disulfide
/
Chromatography, High Pressure Liquid
/
Thiazolidines
/
Methods
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2012
Type:
Article
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