Effect comparison of Wright-Giemsa stain under different conditions and its apply in cell micronucleus test / 中华劳动卫生职业病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To apply the Wright-Giemsa stain in micronucleus test and to explore the stain outcomes of Wright-Giemsa dye of various proportions and staining times.</p><p><b>METHODS</b>Use Wright-Giemsa dye, Wright dye (staining time 3 min) and Giemsa dye (staining time 5 min) to stain HepG2 and then observe the staining effect. The Wright-Giemsa dye was applied under 5 different proportions (31-13) and different staining times (1, 3, 5, 10, 15 min).</p><p><b>RESULTS</b>After stained for 3-5 min with the proportion ratio of 31 of Wright-Giemsa dye, the HepG2 cells showed much better staining outcomes compared with the single stain of either Wright or Giemsa.</p><p><b>CONCLUSIONS</b>Wright-Giemsa stain can be used in cell micronucleus test to obtain good staining outcomes.</p>