Tissue culture and plant regeneration of Ixeridium sonchifolium / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the callus induction and plant regeneration of Ixeridium sonchifolium.</p><p><b>METHOD</b>By using the orthogonal experiment design, the medium for callus induction and plant regeneration was optimized.</p><p><b>RESULT</b>The optimal medium for callus induction was MS + 2, 4-D 1.5 mg x L(-1) +6-BA 1.5 mg x L(-1) +NAA 1.0 mg x L(-1) + IBA 1.5 mg x L(-1) + KT1.5 mg x L(-1), the optimal medium for inducing adventitious bud was MS +2, 4-D 0.2 mg x L(-1) +6-BA 0.5 mg x L(-1) + NAA 0.5 mg x L(-1) + IBA 0.5 mg x L(-1) + KT 0.5 mg x L(-1). Plantlets were rooted on 1/4MS medium supplemented with different concentrations of IBA, and high rooting and survival was achieved when the IBA concentration was 0.1 mg L(-1).</p><p><b>CONCLUSION</b>An efficient system for plant regeneration of I. sonchifolium was established.</p>