Determination of rubrofusarin gentiobioside in Cassia obtusifolia by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 366-368, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-324283
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for determining the content of rubrofusarin gentiobioside in Cassia obtusifolia.</p><p><b>METHOD</b>The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, acetonitrile-THF-1% acetic acid (18 379) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees 2 and detection wavelength at 278 nm.</p><p><b>RESULT</b>A good linearity was obtained from 0.1-0.5 microg with r = 0.999 9 for rubrofusarin gentiobioside. The average recovery was 101.1%, and RSD was 2.23% (n = 5).</p><p><b>CONCLUSION</b>The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Drugs, Chinese Herbal
/
Cassia
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Chromones
/
Glucosides
/
Methods
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS