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Determination of five primary chemical constituents in Cassia angustifolia by HPLC / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-324284
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for determining the content of primary chemical constituents in the leaves of Cassia angustifolia.</p><p><b>METHOD</b>The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used , acetonitrile-1% acetic acid (1090-15 85-18 82-20 80-25 75) in a gradient manner was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees C and detection wavelength at 270 nm.</p><p><b>RESULT</b>The results showed that 5 effective components all separated well and showed good linearity.</p><p><b>CONCLUSION</b>The method was proved to be rapid, sensitive, accurate, credible and repeatable. It can be applied to quality control of Folium Sennae.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Senna Extract / Temperature / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid / Anthraquinones / Senna Plant / Apigenin / Glucosides / Methods Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Senna Extract / Temperature / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid / Anthraquinones / Senna Plant / Apigenin / Glucosides / Methods Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2008 Type: Article