Research on phytoestrogenic effects and its mechanism of psoralen / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 59-63, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-324298
ABSTRACT
<p><b>OBJECTIVE</b>To observe and evaluate the phytoestrogenic effects and its mechanism of psoralen in estrogen receptor (ER) alpha and beta positive T47D and ishikawa cells.</p><p><b>METHOD</b>The proliferation rate of T47D influenced by 1 x 10(-5) mol x L(-1) to 1 x 10(-9) mol x L(-1) psoralen and that of Ishikawa influenced by 1 x 10(-6) mol x L(-1) and 1 x 10(-7) mol x L(-1) psoralen were analyzed by MTT assay. PR mRNA expression in T47D was quantified by RT-PCR assay. Estrogen receptor antagonist ICI 182, 780 was employed as a tool. ER-alpha and ER-beta expression of T47D was measured by flow cytometry.</p><p><b>RESULT</b>The proliferation rates of T47D cells treated with 1 x 10(-5) mol x L(-1) to 1 x 10(-7) mol x L(-1) psoralen and ishikawa cells treated with 1 x 10(-6) mol x L(-1) to 1 x 10(-7) mol x L(-1) psoralen were increased significantly. The RT-PCR result showed that 1 x 10(-7) mol x L(-1) and 1 x 10(-6) mol x L(-1) psoralen could increase PR expression in T47D cells. The above effects could be blocked by ICI 182,780. Psoralen could also induce the augment of ER-alpha and ER-beta expression in T47D cells significantly.</p><p><b>CONCLUSION</b>Psoralen has phytoestrogenic effects. The effects are attained through ER pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Receptors, Progesterone
/
Receptors, Estrogen
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Line, Tumor
/
Cell Proliferation
/
Estradiol
/
Ficusin
/
Flow Cytometry
/
Genetics
Limits:
Female
/
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2008
Type:
Article
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