Preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography with macroporous resin / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2595-2597, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-324326
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for the preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography (HSCCC).</p><p><b>METHOD</b>The crude alcohol extracts were eluted on a macroporous resin column and then purified by high speed counter-current chromatography (HSCCC). A two-phase solvent system composed of ethyl acetate n-butanol water (2 1 3) was used, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL x min(-1), while the apparatus rotated at 800 r x min(-1) and the eluate was detected at 254 nm.</p><p><b>RESULT</b>136 mg gentiopicrin with purity of 99.6% determined by HPLC were obtained from 300 mg crude extraction only in one-step separation and less than 200 minutes.</p><p><b>CONCLUSION</b>The established method is simple, high efficiency and suitable for large-scale separation of gentiopicrin.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Resins, Synthetic
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Plant Roots
/
Countercurrent Distribution
/
Gentiana
/
Rhizome
/
Iridoids
/
Iridoid Glucosides
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2007
Type:
Article
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